Device

Part:BBa_K3629013:Design

Designed by: Sravya Kakumanu   Group: iGEM20_Calgary   (2020-10-18)
Revision as of 07:13, 18 October 2020 by Sravyakakumanu (Talk | contribs) (→‎Source)


Modified PfCBHI expression construct with gibson homology sequences and 6X His tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2725
    Illegal EcoRI site found at 2546
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 2546
    Illegal NheI site found at 75
    Illegal SpeI site found at 2726
    Illegal PstI site found at 2740
    Illegal NotI site found at 7
    Illegal NotI site found at 2733
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 2546
    Illegal BglII site found at 1322
    Illegal BamHI site found at 2631
    Illegal XhoI site found at 87
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2726
    Illegal EcoRI site found at 2546
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 2546
    Illegal XbaI site found at 16
    Illegal SpeI site found at 2726
    Illegal PstI site found at 2740
    Illegal NgoMIV site found at 804
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed for Gibson Assembly


Source

The coding sequence of the CBHI is from Penicillium funiculosum, however it has been codon-optimized and mutated to have optimal activity in Yarrowia lipolytica based on our modelling. The promoter and terminator sequences are from wild-type Y. lipolytica.

References