Device

Part:BBa_K3629013:Design

Designed by: Sravya Kakumanu   Group: iGEM20_Calgary   (2020-10-18)
Revision as of 07:11, 18 October 2020 by Sravyakakumanu (Talk | contribs) (→‎Source)


Modified PfCBHI expression construct with gibson homology sequences and 6X His tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2725
    Illegal EcoRI site found at 2546
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 2546
    Illegal NheI site found at 75
    Illegal SpeI site found at 2726
    Illegal PstI site found at 2740
    Illegal NotI site found at 7
    Illegal NotI site found at 2733
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 2546
    Illegal BglII site found at 1322
    Illegal BamHI site found at 2631
    Illegal XhoI site found at 87
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2726
    Illegal EcoRI site found at 2546
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 2546
    Illegal XbaI site found at 16
    Illegal SpeI site found at 2726
    Illegal PstI site found at 2740
    Illegal NgoMIV site found at 804
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed for Gibson Assembly


Source

The coding sequence of the CBHI is from Penicillium funiculosum, however it has been modified to have optimal activity in Yarrowia lipolytica based on our modelling. The promoter and terminator sequences are from wild-type Y. lipolytica .

References