Coding

Part:BBa_K3352001:Design

Designed by: Joyce Ting   Group: iGEM20_TAS_Taipei   (2020-10-18)
Revision as of 05:08, 18 October 2020 by Registry (Talk | contribs)

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Φ29 DNA Polymerase with His-Tag and GS linker Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 595
    Illegal SapI.rc site found at 993


Design Notes

We attached a 6x his-tag upstream of the phi29 DNA Polymerase for purification purposes followed by a glycine-serine linker (GS linker) to form our ORF.


Source

We obtained the amino acid sequence of phi29 DNA polymerase from Bacillus phage phi29.

References