Device

Part:BBa_K3629015

Designed by: Sravya Kakumanu   Group: iGEM20_Calgary   (2020-10-17)
Revision as of 04:18, 18 October 2020 by Registry (Talk | contribs)

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T. reesei CBHII expression construct with gibson homology sequences and FLAG tag

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Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2737
    Illegal EcoRI site found at 2561
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 2561
    Illegal NheI site found at 75
    Illegal SpeI site found at 2738
    Illegal PstI site found at 2752
    Illegal NotI site found at 7
    Illegal NotI site found at 2745
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 2561
    Illegal BamHI site found at 2686
    Illegal XhoI site found at 124
    Illegal XhoI site found at 1198
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2738
    Illegal EcoRI site found at 2561
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 2561
    Illegal XbaI site found at 16
    Illegal SpeI site found at 2738
    Illegal PstI site found at 2752
    Illegal NgoMIV site found at 1458
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None