![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3332053
Designed by: Yangqi Deng Group: iGEM20_XMU-China (2020-10-17)
J23100-RBS-Lpp-OmpA-GOX-terminator
We anchored GOX protein onto membranes through Lpp-OmpA to catalyze the reaction of glyphosate to form glyoxalic acid and AMPA and add his-tag to purify the protein. We use K880005 to construct the expression system and anchor GOX on the surface of E.coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 820
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1039
Illegal AgeI site found at 1109
Illegal AgeI site found at 1186 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |