Composite

Part:BBa_K3332053

Designed by: Yangqi Deng   Group: iGEM20_XMU-China   (2020-10-17)
Revision as of 21:08, 17 October 2020 by Registry (Talk | contribs)

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J23100-RBS-Lpp-OmpA-GOX-terminator

We anchored GOX protein onto membranes through Lpp-OmpA to catalyze the reaction of glyphosate to form glyoxalic acid and AMPA and add his-tag to purify the protein. We use K880005 to construct the expression system and anchor GOX on the surface of E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 820
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1039
    Illegal AgeI site found at 1109
    Illegal AgeI site found at 1186
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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