Coding
EcAKR4-1-B

Part:BBa_K3332010

Designed by: Xiaoyu Wang   Group: iGEM20_XMU-China   (2020-10-17)
Revision as of 20:37, 17 October 2020 by Registry (Talk | contribs)

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GOX-BrkA

We anchor GOX protein onto membranes through BrkA to catalyze the reaction of degradating glyphosate to form glyoxalic acid and AMPA. We use K880005 to construct the expression system and anchor GOX on the surface of E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 294
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 513
    Illegal NgoMIV site found at 1243
    Illegal NgoMIV site found at 1672
    Illegal NgoMIV site found at 2308
    Illegal AgeI site found at 583
    Illegal AgeI site found at 660
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2276


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