![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
EcAKR4-1-B
Part:BBa_K3332010
Designed by: Xiaoyu Wang Group: iGEM20_XMU-China (2020-10-17)
GOX-BrkA
We anchor GOX protein onto membranes through BrkA to catalyze the reaction of degradating glyphosate to form glyoxalic acid and AMPA. We use K880005 to construct the expression system and anchor GOX on the surface of E.coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 294
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 513
Illegal NgoMIV site found at 1243
Illegal NgoMIV site found at 1672
Illegal NgoMIV site found at 2308
Illegal AgeI site found at 583
Illegal AgeI site found at 660 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2276
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Categories
Parameters
None |