Protein_Domain

Part:BBa_K103013:Design

Designed by: Michael Lower   Group: iGEM08_Warsaw   (2008-10-09)
Revision as of 14:16, 28 October 2008 by Smaegol (Talk | contribs) (→‎Design Notes)

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linker fused to omega fragment of TEM beta-lactamase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 190


Design Notes

Preparation of BBa_K103013 is in details described [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=6&arg0=18_September_2008&arg1=2_October_2008&arg2=3_October_2008&arg3=6_October_2008&arg4=7_October_2008&arg5=8_October_2008&name=Preparation%20of%20linker_omega%20%28BBa_K103013%29 here] (entries from Univeristy of Warsaw 2008 iGEM team notebook).

Omega_linker was amplified from [http://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega pACYC177+OmpA-omega] vector using primers: 5' ATGAATTCGCGGCCGCTTCTAGAGGGAGCTCTGGTGGAA GCGGTGGCGGAGGATC 3'and 5' GCACTAGTATTACCAATGCTTAATCAGTGAG 3'. Resulting fragment was digested with EcoRI and BcuI and ligated into standard BioBrick vector (pSB2K3)

Source

References