Composite

Part:BBa_K3332050

Designed by: Shengyang Zhang   Group: iGEM20_XMU-China   (2020-10-17)
Revision as of 19:36, 17 October 2020 by Registry (Talk | contribs)

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J23100-RBS-iNAP-BrkA-terminator

iNAP is fused at C-terminal with BrkA anchoring protein. We use K880005 to construct the expression system and anchor iNAP on the surface of E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 333
    Illegal XhoI site found at 359
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 99
    Illegal NgoMIV site found at 1520
    Illegal NgoMIV site found at 1949
    Illegal NgoMIV site found at 2585
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2553


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Categories
Parameters
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