Coding

Part:BBa_K103004:Design

Designed by: Michael Lower   Group: iGEM08_Warsaw   (2008-09-14)
Revision as of 14:03, 28 October 2008 by Smaegol (Talk | contribs) (→‎Design Notes)

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protein ZSPA-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Artificially synthesized (thanks to [http://www.geneart.com/index.php?id=284 Geneart] help). The coding sequence is totally different from original, because of optimized codon usage.

Detailed information about preparation of this brick could be find [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=4&arg0=29_September_2008&arg1=30_September_2008&arg2=1_October_2008&arg3=2_October_2008&name=Preparation%20of%20Z%28BBa_K103004%29 here] (entries from Univeristy of Warsaw 2008 iGEM team notebook)

Protein Z coding sequence was cutted from [http://2008.igem.org/Wiki/Team:Warsaw/vectors/pGeneart_Z pGeneart+Z] with NdeI and BcuI and ligated into pSB1A3 (pSB1A3 with NdeI site was obtained by SacI/BcuII digest of pSB1A3 carrying BBa_K103003)

Source

[http://www.ncbi.nlm.nih.gov/pubmed/15238637?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum]

References