Composite

Part:BBa_K3409010

Designed by: Albane Mabro   Group: iGEM20_Ionis_Paris   (2020-10-17)
Revision as of 18:57, 17 October 2020 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Microcin PDI cluster genes for McpM, McpI, McpA

Contains the elements necessary to express the three first genes from the Microcin PDI gene cluster (mcpM: coding for microcin PDI, mcpI: coding for the immunity protein, mcpA: coding for the activator protein). All three genes are under the control of the same promoter and the sequence is organized as follows: a promoter (BBa_J23111), an RBS (BBa_J61127), the CDS for McpM-His (BBa_K3409003), an RBS (BBa_J61130), the CDS for McpI (BBa_K3409004), an RBS (BBa_J61118), the CDS for McpA (BBa_K3409005) and a double forward terminator (BBa_B0015). Note that it must be expressed with the two other genes from the cluster (mcpD and mcpB) for secretion of McpM. Will constitutively generate McpM, McpI and McpA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None