Coding
iNAP

Part:BBa_K3332000:Design

Designed by: Shuling Xiao   Group: iGEM20_XMU-China   (2020-10-17)
Revision as of 18:15, 17 October 2020 by Jisheng Xie (Talk | contribs)

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iNAP-his-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 272
    Illegal XhoI site found at 298
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 38
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The iNAP sequence we use is different from what we mentioned in this part. If you have any questions about it, please contact with us via email igem_xmu@163.com.


Source

Circularly permuted YFP(cpYFP)and Rex from Thermus aquaticus(T-Rex). We ordered the part DNA from Genscript® company along with CDS below.

References