Generator

Part:BBa_K3527003

Designed by: Kong Yangyang   Group: iGEM20_Shanghai_HS   (2020-10-17)
Revision as of 17:32, 17 October 2020 by Registry (Talk | contribs)

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PhoA-signal peptide-EGFP

In E. coli, the expression level of PhoA gene is affected by the concentration of phosphate. BBa_K3527003 uses enhancer green fluorescence protein (EGFP) gene as the reporter gene to construct the fusion detection vector PhoA-EGFP, and then the sythetic generator can be used as the biosensor displays different fluorescence intensities according to the level of phosphorus content in the water, which can easily and quickly detect the content of phosphorus in different water bodies

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None