Protein_Domain

Part:BBa_K3373007:Design

Designed by: Antonin Chevenier   Group: iGEM20_Sorbonne_U_Paris   (2020-10-17)
Revision as of 17:23, 17 October 2020 by Registry (Talk | contribs)

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TMD-HAP2 codon optimized for C. reinhardtii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The CDS is standardized in the Phytobrick standard for the C. reinhardtii MoClo Kit at the B3 (N-terminus coding region) position by adding BbsI sites and the corresponding fusion sites on both ends. The start codon was added The stop codon was removed to allow for tagging at the C-terminal position, the stop codon being provided by the part on the position B5.



Source

Chlamydomonas reinhardtii 3055 [NCBI]

References