Plasmid

Part:BBa_K3394009:Design

Designed by: Talia Shaler   Group: iGEM20_BGU-Israel   (2020-10-17)
Revision as of 16:25, 17 October 2020 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


ICE (Integrative and Conjugative Element)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 13962
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2017
    Illegal BsaI.rc site found at 13388


Design Notes

Most ICE consist a large plasmid that may "overload" our bacteria. Therfore tried to find a "mini" ICE system.


Source

Artificial sequence - Synthetic construct

References