Composite

Part:BBa_K3332074

Designed by: Siru Zhou   Group: iGEM20_XMU-China   (2020-10-17)
Revision as of 15:33, 17 October 2020 by Registry (Talk | contribs)

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J23101-OmpA 5'UTR-phnF 0.85-Hfq binding sequence-J61048

Encoding the siRNA squence which can silence the phnF gene in E.coli BL21(DE3).Use K823005 to silence the phnF gene in E.coli BL21 (DE3).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 281
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 294
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None