Composite

Part:BBa_K3332068

Designed by: Tianyu Feng   Group: iGEM20_XMU-China   (2020-10-17)
Revision as of 15:09, 17 October 2020 by Registry (Talk | contribs)

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J23100-RBS-phnJ-terminator

Use BBa_K823004-B0034 to express the subunit of C-P lyase, which can degrade the glyphosate, from Enterobacterales. This subunit is essential to the activity of the whole enzyme.Improve the capability of chassis bacteria to degrade glyphosate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 174
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 658
    Illegal NgoMIV site found at 680
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 670


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Categories
Parameters
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