Coding

Part:BBa_K3332025

Designed by: Zhichun Yang   Group: iGEM20_XMU-China   (2020-10-17)
Revision as of 14:36, 17 October 2020 by Registry (Talk | contribs)

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phnJ_mut21

The mutant of C-P lyase, which can degrade the glyphosate, from Enterobacterales. This subunit is essential to the activity of the whole enzyme.Use K823004 to construct a new part that can improve the capability of chassis bacteria to degrade glyphosate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 549
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 597
    Illegal AgeI site found at 268
    Illegal AgeI site found at 775
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None