Part:BBa_K103003:Design
B domain of Staphylococcal protein A
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 91
Illegal BamHI site found at 9 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Preparation of BBa_K103003 is in details described [http://2008.igem.org/wiki/index.php?title=Team:Warsaw/JSTest&num=7&arg0=18_September_2008&arg1=19_September_2008&arg2=22_September_2008&arg3=23_September_2008&arg4=24_September_2008&arg5=25_September_2008&arg6=26_September_2008&name=Preparation%20of%20%26Delta%3BA%20%28BBa_K103003%29 here] (entries from Univeristy of Warsaw 2008 iGEM team notebook).
B-domain of Staphylococcal protein A was amplified from [http://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega-A-alpha pACYC177+OmpA-omega-ΔA-alpha] vector using primers: 5' TAGAATTCGCGGCCGCTTCTAGA GGGCATATGGGATCCAAAACCGCGGCTCTTGCGCAAC 3' and 5' GGACTAGTAGAGCTCCCGTCTACTTTCGGCGCCTGAGC 3'. Resulting fragment was then digested with EcoRI and BcuI and cloned into standard BioBrick vector pSB1A3