Composite

Part:BBa_K3470004:Design

Designed by: Shravan Balasubramaniam   Group: iGEM20_MIT_MAHE   (2020-10-17)
Revision as of 09:26, 17 October 2020 by Registry (Talk | contribs)

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Modified mer Operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 652
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1878
    Illegal NgoMIV site found at 3162
    Illegal NgoMIV site found at 3210
    Illegal NgoMIV site found at 3272
    Illegal NgoMIV site found at 3483
    Illegal NgoMIV site found at 4088
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4982
    Illegal SapI site found at 637


Design Notes

Gibson Assembly for assembly of parts


Source

E.coli DH5alpha

References