Coding
Part:BBa_K3373005
Designed by: Antonin Chevenier Group: iGEM20_Sorbonne_U_Paris (2020-10-16)
UVR8 A. thaliana codon optimized for C. reinhardtii
Coding sequence of the Ultraviolet-B receptor encoded by the gene UVR8 from Arabidopsis thaliana and codon optimized for Chlamydomonas reinhardtii. Upon UV-B irradiation, UVR8 undergoes an immediate switch from homodimer to monomer wich lead to the interaction with the photomorphogenic repressor COP1.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 28
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 28
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 850
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 28
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 28
Illegal NgoMIV site found at 75
Illegal AgeI site found at 672 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |