Coding

Part:BBa_K3373004

Designed by: Antonin Chevenier   Group: iGEM20_Sorbonne_U_Paris   (2020-10-16)
Revision as of 22:55, 16 October 2020 by Registry (Talk | contribs)

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COP1 A. thaliana codon optimized for C. reinhardtii

Coding sequence of the E3 ubiquitin-protein ligase COP1 (P43254) encoded by the gene COP1 from Arabidopsis thaliana and codon optimized for Chlamydomonas reinhardtii. This enzyme is involved in the protein ubiquitination pathway. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin conjugating enzyme and then directly transfers the ubiquitin to targeted substrates for proteasomal degradation.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 399
    Illegal PstI site found at 576
    Illegal PstI site found at 924
    Illegal PstI site found at 939
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 399
    Illegal PstI site found at 576
    Illegal PstI site found at 924
    Illegal PstI site found at 939
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 399
    Illegal PstI site found at 576
    Illegal PstI site found at 924
    Illegal PstI site found at 939
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 399
    Illegal PstI site found at 576
    Illegal PstI site found at 924
    Illegal PstI site found at 939
    Illegal NgoMIV site found at 1229
    Illegal NgoMIV site found at 1935
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None