Coding
Cr-atzA B3

Part:BBa_K3373001:Design

Designed by: Antonin Chevenier   Group: iGEM20_Sorbonne_U_Paris   (2020-10-11)
Revision as of 22:14, 16 October 2020 by Antonin chev (Talk | contribs) (→‎References)

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atzA P. sp. ADP codon optimized for C. reinhardtii


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 825
    Illegal PstI site found at 1389
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 825
    Illegal PstI site found at 1389
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 825
    Illegal PstI site found at 1389
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 825
    Illegal PstI site found at 1389
    Illegal NgoMIV site found at 253
    Illegal NgoMIV site found at 653
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The CDS is standardized in the Phytobrick standard for the C. reinhardtii MoClo Kit at the B3-B4 position by adding BbsI sites and the corresponding fusion sites on both ends.

The stop codon was removed to allow for tagging at the C-terminal position, the stop codon being provided by the part on the position B5.


Source

Pseudomonas sp. ADP strain.

References

1. Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.

2. Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. A modular cloning system for standardized assembly of multigene constructs. PLoS One. 2011 Feb 18;6(2):e16765. doi: 10.1371/journal.pone.0016765. PMID: 21364738; PMCID: PMC3041749.

3. de Souza ML, Wackett LP, Boundy-Mills KL, Mandelbaum RT, Sadowsky MJ. Cloning, characterization, and expression of a gene region from Pseudomonas sp. strain ADP involved in the dechlorination of atrazine. Appl Environ Microbiol. 1995 Sep;61(9):3373-8. doi: 10.1128/AEM.61.9.3373-3378.1995. PMID: 7574646; PMCID: PMC167616.