Other
Part:BBa_K3425030:Design
Designed by: Núria Garriga Alonso Group: iGEM20_UofUppsala (2020-10-16)
iGEM Type IIS pSB3C14-DY
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Experiments with cloning the original dummy sequences TU-DY (<a href="parts.igem.org/Part:BBa_K3425021">BBa_K3425021</a>) and MTU-DY (<a href="parts.igem.org/Part:BBa_K3425022">BBa_K3425022</a>) together (<a href="parts.igem.org/Part:BBa_K3425064">BBa_K3425064</a> and <a href="parts.igem.org/Part:BBa_K3425065">BBa_K3425065</a>) showed that these parts should be longer than 4bp and different from each other. This is reflected in the design of the new set of dummies, which is 8bp long and all of them are different.
Source
Random DNA generator (https://www.faculty.ucr.edu/~mmaduro/random.htm)