Generator

Part:BBa_K103019

Designed by: Michael Lower   Group: iGEM08_Warsaw   (2008-10-09)
Revision as of 12:54, 28 October 2008 by Smaegol (Talk | contribs)

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alpha_linker under PT7



Usage and Biology

  • Construct for creating prey fusions for our 'hunter and prey' selection system
  • Fusion partners are connected via Gly-Ser-Gly linker
  • Prey protein should be attached via linker (using BamHI+ Biobrick standard suffix enzyme).
  • Fusion protein can be purified on NiNTA bead (tkanks to presence of N-terminal His-Tag).
  • Generator under control of T7 promoter
  • Interaction between hunter and prey added to the medium makes cells ampicillin resistant
  • This part uses our NdeI/SacI/BamHI fusion cloning substandard


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None