Part:BBa_K3408010

P_nar-B0034-Trigger RNA-B0015-P_CⅠ-Switch RNA-mazF-B0015

We designed this composite part by combining promoter P_nar, trigger RNA, P_CⅠ, switch RNA and mazF. So we could utilize it to verify the feasibility of our device, which comprises of Toehold switch and suicide protein—mazF. Former composite part BBa_K3408006 has demonstrated the feasibility of promoter P_nar, so when our engineered bacteria Bacillus subtilis is in anaerobic environment, promoter P_nar is activated by global regulator—FNR. Then downstream trigger RNA is expressed. As switch RNA is constantly expressed under the control of promoter P_C1, the trigger RNA can successfully trigger the Toehold switch, thus leading to the expression of mazF. So Bacillus subtilis can commit suicide. While in aerobic environment, promoter P_nar ceases to work, Toehold switch cannot be triggered. As a result, mazF can not express. This device proves that our suicide module and Toehold switch can successfully combine together.

Plasmid profile

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 187
  Illegal BglII site found at 409
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]