Composite

Part:BBa_K3408007

Designed by: Chen Peng   Group: iGEM20_NAU-CHINA   (2020-10-06)
Revision as of 13:44, 16 October 2020 by NJAU-Chappie (Talk | contribs)


Pnar-B0034-phy(ycD)-B0015

Used Pnar(BBa_K3408000), RBS(BBa_B0034), phy(ycD)(BBa_E0040) and terminator(BBa_B0015) to express Phy(ycD) under the control of Pnar. When our Bacillus subtilis was in an anerobic environment, Pnar was activated by FNR so that Phy(ycD) could be expressed. But when our Bacillus subtilis was in an aerobic environment, Pnar could be suppressed and we couldn’t detect more Phy(ycD).

Plasmid profile T--NAU-CHINA--composite-parts1.1.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 454
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None