Composite

Part:BBa_K3332091

Designed by: Ruijie Mo   Group: iGEM20_XMU-China   (2020-10-16)
Revision as of 12:30, 16 October 2020 by Registry (Talk | contribs)

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Formaldehyde_derivative-1 promoter-ECFP-terminator

An improvement of BBa_K1334002 by enhancing the strength of the component "weak promoter". It's more sensitive to formaldehyde.wE Use it to degrade LacI protein tagged with ssrAtag(mf-lon).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 422
    Illegal NheI site found at 445
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
abs
biology
colorCyan
directionForward
emissionCFP
emit476
excitation
excite439
kegg
lum
proteinECFP
swisspro
tagNone