RNA

Part:BBa_K115002:Design

Designed by: Bastiaan van den Berg   Group: iGEM08_TUDelft   (2008-07-15)
Revision as of 12:40, 28 October 2008 by Bavandenberg (Talk | contribs) (→‎Source)

RNA thermometer (FourU)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The figure shows the secondary structure of the original RNA thermometer as well as the secondary structure of this part. Because of the scar that is introduced during the ligation process, some nucleotides had to be changed in order to get the same secondary structure as that of the original one. The blue nucleotides are the ones that are being changed. The working of the RNA thermometer is based on this secondary structure and that is why it is important that this part has the same secondary structure (after ligation to a protein coding part) as the original RNA thermometer.

More information on the design of this part can be found [http://2008.igem.org/Team:TUDelft/Temperature_design here].

BBa K115002.png

Source

This sequence is taken from the Salmonella Enterica Tyhpy ([http://www.ncbi.nlm.nih.gov/sites/entrez?Db=genome&Cmd=ShowDetailView&TermToSearch=21688 NC_010102])) as the 5'UTR fourU RNA thermometer of a heat shock protein.

References

Torsten Waldminghaus, Nadja Heidrich, Sabine Brantl, and Franz Narberhaus. FourU: a novel type of RNA thermometer in Salmonella. Molecular Microbiology, 65(2):413-424, 2007. [http://www.ncbi.nlm.nih.gov/pubmed/17630972 PMID:17630972]