Regulatory

Part:BBa_K3598000

Designed by: Ruijuan Xiang   Group: iGEM20_Beijing_4ELEVEN   (2020-10-05)
Revision as of 07:53, 16 October 2020 by Ruijuan Xiang (Talk | contribs)


AOX1 promoter

AOX1 is a very common inducible promoter in yeast, and its ON/OFF condition is controlled by methanol.


Usage and Biology

We added AOX1 promoter and terminator into a plasmid in Pichia pastoris containing the sequence of sfGFP and recorded the intensity of the expression of fluorescence proteins in our experiment. We added 5% methanol into the culture for 5 days, and extracted the supernatant and ran 4%-20% SDS-PAGE gel for every 24 hours.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 937
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None