Composite

Part:BBa_K3610045

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-10-08)
Revision as of 20:57, 15 October 2020 by Jtrott (Talk | contribs)


EFR ectodomain / YFP

This part contains the ectodomain of the plant cell surface receptor from A. thaliana fused to a yellow fluorescent protein. This part lacks the natural N-terminal signal sequence but instead uses the signal sequence from the alpha-Factor from yeast.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 355
    Illegal NheI site found at 1285
    Illegal NheI site found at 2203
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1871
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None