Composite

Part:BBa_K3610041:Design

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-10-10)
Revision as of 20:39, 15 October 2020 by Jtrott (Talk | contribs) (→‎References)

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EFR ectodomain / mCherry N-terminal for C. reinhardtii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 93
    Illegal NheI site found at 235
    Illegal NheI site found at 1165
    Illegal NheI site found at 2083
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 148
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1751
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

All codon optimization was done with SnapGene

Source

BBa_K2136017, BBa_K3610012, BBa_K3610008, BBa_K416001, BBa_K1150016, BBa_K3610018

References

Fan, Jin-Yu; Cui, Zong-Qiang; Wei, Hong-Ping; Zhang, Zhi-Ping; Zhou, Ya-Feng; Wang, Yun-Peng; Zhang, Xian-En (2008): Split mCherry as a new red bimolecular fluorescence complementation system for visualizing protein–protein interactions in living cells. In: Biochemical and Biophysical Research Communications 367 (1), S. 47–53. DOI: 10.1016/j.bbrc.2007.12.101.

Kunze, Gernot; Zipfel, Cyril; Robatzek, Silke; Niehaus, Karsten; Boller, Thomas; Felix, Georg (2004): The N terminus of bacterial elongation factor Tu elicits innate immunity in Arabidopsis plants. In: Plant Cell 16 (12), S. 3496–3507. DOI: 10.1105/tpc.104.026765.