Coding

Part:BBa_K3504013

Designed by: Ahmed Wael   Group: iGEM20_AFCM-Egypt   (2020-10-15)
Revision as of 10:32, 15 October 2020 by Registry (Talk | contribs)

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nSP1capping-semliki forest virus

Nsp1 is one of four non structural proteins that together forms the main complex responsible for the synthesis positive-sense viral RNAs, results in the synthesis of both the genomic and subgenomic RNAs, of which the subgenomic RNA is produced in excess of the viral genome. Which allows the virus to self-replicate into millions of copies of the virus.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 358
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 564
    Illegal PstI site found at 358
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 358
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 358
    Illegal NgoMIV site found at 1551
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None