Coding

Part:BBa_K3394001

Designed by: Talia Shaler   Group: iGEM20_BGU-Israel   (2020-10-11)
Revision as of 07:33, 15 October 2020 by Talia sh (Talk | contribs)


Coding Sequence of CBD (Cellulose Binding Domain)

Most cellulolytic complexes consist of catalytic domains (Cellulases) and Cellulose-Binding Domains (CBDs).


The binding domain improves the attachment of the enzyme to the substrate and facilitate the catalytic domain activity. Similar binding domain structures can be found in complexes degrading other insoluble carbohydrates such as chitin.

Figure 1: CBD - Cellulose-Binding Domains

The CBD sequence we used was cloned into an Escherichia coli expression vector (PET-28) in the lab.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 37
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 37
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 37
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 37
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 37
    Illegal AgeI site found at 12
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None