Composite

Part:BBa_K3585001:Design

Designed by: Kong Yangyang   Group: iGEM20_Shanghai_HS_United   (2020-10-14)
Revision as of 19:27, 14 October 2020 by Kimmy2020 (Talk | contribs) (→‎Design Notes)

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Biosynthetic gene cluster of butyrate synthesis pathway enzymes


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1175
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6207
    Illegal BglII site found at 7343
    Illegal BglII site found at 7484
    Illegal XhoI site found at 1180
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to meet the typeIIS standard, we made a synonymous mutation of Asp50Asp and Leu137Leu to remove BsaI restriction site.

Source

It comes from the genomic sequence of Clostridium beijerinckii.

References