![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3610034
Designed by: Jonas Sebastian Trottmann Group: iGEM20_UZurich (2020-10-05)
BAK1 ectodomain / mCherry N-terminal
This part contains the sequence for the ectodomain of the plant surface receptor BAK1 fused to the N-terminal part of a split-mCherry protein. Additionally, instead of the signal peptide native to the plant receptor, there is the secretion signal of the alpha factor from yeast at the N-terminal domain of the receptor, replacing the original signal sequence.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 849
Illegal PstI site found at 894 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 849
Illegal PstI site found at 894 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 849
Illegal PstI site found at 894 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 849
Illegal PstI site found at 894 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |