Composite

Part:BBa_K3610034

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-10-05)
Revision as of 15:02, 14 October 2020 by Jtrott (Talk | contribs)


BAK1 ectodomain / mCherry N-terminal

This part contains the sequence for the ectodomain of the plant surface receptor BAK1 fused to the N-terminal part of a split-mCherry protein. Additionally, instead of the signal peptide native to the plant receptor, there is the secretion signal of the alpha factor from yeast at the N-terminal domain of the receptor, replacing the original signal sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 849
    Illegal PstI site found at 894
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 849
    Illegal PstI site found at 894
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 849
    Illegal PstI site found at 894
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 849
    Illegal PstI site found at 894
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None