Coding

Part:BBa_K3610005

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-08-24)
Revision as of 14:50, 14 October 2020 by Jtrott (Talk | contribs)


EFR from A. thaliana

This part entails the sequence of the plant PRR EFR from A. thaliana.

Usage and Biology

Elongation factor-thermo unstable receptor (EFR) from A. thaliana is a plant pattern-recognition receptor (PRR). It is a cell surface receptor and part of the plants firts defence mechanism against potential pathogens. The EFR receptor is also a leucin-rich-repeats (LRR) receptor-like serine/threonine-protein kinase. The protein consists of an extracellular domain with leucin-rich repeats, a ligand binding domain found in many receptors, a single-pass transmembrane domain and finally an intracellular kinase domain. The ligand binding domain from EFR has high specificity to a bacterial pathogen-associated moleculat pattern (PAMP), namely the epitope elf18 of the abundant protein Elongation Factor Tu (EF-Tu), which is catalyzes the binding of aminoacyl-tRNA (aa-tRNA) to the ribosome in most prokaryotes and therefore is evolutionarily highly conserved. This makes the EFR a receptor that can be activated by the presence of a huge variety of bacteria. Upon binding of the ligand to the extracellular domain, the receptor dimerizes with its coreceptor BRI1-associated receptor kinase (BAK1). This interaction triggers the activation of the intracellular kinase domain of EFR and BAK1, initiating a signal cascade leading to an upregulation of immune response mechanisms.


In our project we used this receptor to demonstrate expression of plant PRRs in S. cerevisiae by expressing this part with a fluorescent marker fused to the N-terminal end of the sequence. We further wanted to see where within the cell the protein localizes.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2753
    Illegal XbaI site found at 433
    Illegal XbaI site found at 2568
    Illegal SpeI site found at 1114
    Illegal PstI site found at 2407
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2753
    Illegal SpeI site found at 1114
    Illegal PstI site found at 2407
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2753
    Illegal BglII site found at 2418
    Illegal BglII site found at 2446
    Illegal BglII site found at 2989
    Illegal XhoI site found at 2442
    Illegal XhoI site found at 3032
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2753
    Illegal XbaI site found at 433
    Illegal XbaI site found at 2568
    Illegal SpeI site found at 1114
    Illegal PstI site found at 2407
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2753
    Illegal XbaI site found at 433
    Illegal XbaI site found at 2568
    Illegal SpeI site found at 1114
    Illegal PstI site found at 2407
    Illegal AgeI site found at 256
  • 1000
    COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None