Part:BBa_K3610032:Design

BAK1 ectodomain / YFP

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 867
  Illegal PstI site found at 912
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 867
  Illegal PstI site found at 912
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 867
  Illegal PstI site found at 912
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 867
  Illegal PstI site found at 912
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

With the native signal peptide removed, the receptor sequence cannot be tranlated in-frame from the start. The scar "ggCAC" between the singal peptide and the receptor encoding domain is important for correct translation. Kinase domain cleaved off after amino acid 287.

Source

BBa_K319005, BBa_J63003, BBa_K2809002, BBa_K3610003, BBa_K416001, BBa_J176006, BBa_K2753050

References

Chinchilla, Delphine; Zipfel, Cyril; Robatzek, Silke; Kemmerling, Birgit; Nürnberger, Thorsten; Jones, Jonathan D. G. et al. (2007): A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence. In: Nature 448 (7152), S. 497–500. DOI: 10.1038/nature05999.

Macho, Alberto P.; Zipfel, Cyril (2014): Plant PRRs and the Activation of Innate Immune Signaling. In: Molecular Cell 54 (2), S. 263–272. DOI: 10.1016/j.molcel.2014.03.028.

Yan, Liming; Ma, Yuanyuan; Liu, Dan; Wei, Xiaochao; Sun, Yuna; Chen, Xiaoyue et al. (2012): Structural basis for the impact of phosphorylation on the activation of plant receptor-like kinase BAK1. In: Cell Res 22 (8), S. 1304–1308. DOI: 10.1038/cr.2012.74.