Part:BBa_K3394001

Coding Sequence of CBD (Cellulose Binding Domain)

Most cellulolytic complexes consist of catalytic domains (Cellulases) and Cellulose-Binding Domains (CBDs).

The binding domain improves the attachment of the enzyme to the substrate and facilitate the catalytic domain activity. Similar binding domain structures can be found in complexes degrading other insoluble carbohydrates such as chitin.

The CBD sequence we used was cloned into an Escherichia coli expression vector (PET-28) in the lab.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 37
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 37
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 37
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 37
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 37
  Illegal AgeI site found at 12
  
• 1000
  COMPATIBLE WITH RFC[1000]