Part:BBa_K3425033

iGEM Type IIS pSB1K03-DY

This part (BBa_K3425025) is an RBS (<a href="https://parts.igem.org/wiki/index.php/Part:BBa_B0030">BBa_B0030</a>) modified for Type IIS iGEM Standard assembly (RFC1000) by adding a five-nucleotide spacer (TAGTA) to the end of the sequence.

Aligned spacing is the distance between the centre of Shine-Dalgarno sequence and ATG start codon (see https://parts.igem.org/Ribosome_Binding_Sites/Design or Ma et al. (2002)).

Five to six nucleotides is the optimal distance between the RBS and the ATG start codon for E. coli (see https://parts.igem.org/Ribosome_Binding_Sites/Design for more general information). Whereas Biobrick assembly generates six-nucleotide scar, Type IIS iGEM Standard assembly generates only one-nucleotide scar between RBS and ATG. To keep the distance optimal, five nucleotides were added to the BBa_B0030 for its use in Type IIS assembly.

Strength of an RBS is dependent on the sequence similarity of

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]