RBS

Part:BBa_K3425033

Designed by: Tereza Hubackova   Group: iGEM20_UofUppsala   (2020-10-13)
Revision as of 08:42, 14 October 2020 by Tereza H (Talk | contribs)


iGEM Type IIS pSB1K03-DY

This part (BBa_K3425025) is an RBS (<a href="https://parts.igem.org/wiki/index.php/Part:BBa_B0030">BBa_B0030</a>) modified for Type IIS iGEM Standard assembly (RFC 1000) by adding a five-nucleotide spacer (TAGTA) to the end of the sequence.

Aligned spacing is the distance between the centre of Shine-Dalgarno sequence and ATG start codon (see https://parts.igem.org/Ribosome_Binding_Sites/Design or Ma et al. (2002)).

Five to six nucleotides is the optimal distance between the RBS and the ATG start codon for E. coli (see https://parts.igem.org/Ribosome_Binding_Sites/Design for more general information). Whereas Biobrick assembly generates six-nucleotide scar, Type IIS iGEM Standard assembly generates only one-nucleotide scar between RBS and ATG. To keep the distance optimal, five nucleotides were added to the BBa_B0030 for its use in Type IIS assembly.



Strength of an RBS is dependent on the sequence similarity of


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//collections
//collections/typeiis/uppsala
//direction/forward
//rbs/prokaryote/constitutive/community
//rbs/prokaryote/constitutive/constitutive
//regulation/constitutive
Parameters
chassisE. coli
directionforward
functionprotein expression regulation
rbsconstitutive