RBS

Part:BBa_K3425033

Designed by: Tereza Hubackova   Group: iGEM20_UofUppsala   (2020-10-13)
Revision as of 21:25, 13 October 2020 by Registry (Talk | contribs)

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iGEM Type IIS pSB1K03-DY

This part (BBa_K3425025) is a strong RBS (BBa_B0030) modified for Type IIS iGEM Standard assembly (RFC 1000) by adding five nucleotides (TAGTA) to the end of the sequence.

Five to six nucleotides is the optimal distance between the RBS and the ATG start codon for E. coli (see https://parts.igem.org/Ribosome_Binding_Sites/Design for more general information). Whereas Biobrick assembly generates six-nucleotide scar, Type IIS iGEM Standard assembly generates only one-nucleotide scar between RBS and ATG. To keep the distance optimal, five nucleotides were added to the BBa_B0030 for its use in Type IIS assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//collections
//collections/typeiis/uppsala
//direction/forward
//rbs/prokaryote/constitutive/community
//rbs/prokaryote/constitutive/constitutive
//regulation/constitutive
Parameters
chassisE. coli
directionforward
functionprotein expression regulation
rbsconstitutive