Plasmid
pUPD2

Part:BBa_K3505007

Designed by: Venetios Michelioudakis   Group: iGEM20_Thessaly   (2020-10-13)
Revision as of 15:54, 13 October 2020 by Venetios (Talk | contribs)


pUPD2 for Golden Braid

Level 0 vector for Golden Braid assembly. The Level 0 modules are Interchangable parts in order everyone can use it as it is without any modification based on Golden Braid grammar.

Usage and Biology

LacZa module as cargo between 2 BsmBI sites and 2 BtgzI sites


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 260
    Illegal EcoRI site found at 2674
    Illegal XbaI site found at 233
    Illegal SpeI site found at 627
    Illegal PstI site found at 221
    Illegal PstI site found at 641
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 260
    Illegal EcoRI site found at 2674
    Illegal SpeI site found at 627
    Illegal PstI site found at 221
    Illegal PstI site found at 641
    Illegal NotI site found at 634
    Illegal NotI site found at 2680
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 260
    Illegal EcoRI site found at 2674
    Illegal BamHI site found at 239
    Illegal XhoI site found at 1658
    Illegal XhoI site found at 2550
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 627
    Illegal EcoRI site found at 260
    Illegal EcoRI site found at 2674
    Illegal XbaI site found at 233
    Illegal PstI site found at 221
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 260
    Illegal EcoRI site found at 2674
    Illegal XbaI site found at 233
    Illegal SpeI site found at 627
    Illegal PstI site found at 221
    Illegal PstI site found at 641
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 14
    Illegal BsaI.rc site found at 611


Functional Parameters

[edit]
Categories
//awards/part_collection
//plasmid/construction
Parameters
None