Part:BBa_K3505006

Tyr1-AIDAc Tyrosinase fused to membrane protein AIDAc. GB compatible B3-B5

Tyrosinase catalyzes the conversion of L-Tyrosine to L-Dopa and L-Dopa quinone. These derivatives can be detected electochemically, producing peak currents.[1] AIDA is native E.coli outer membrane protein. AIDA's CDS constists of 3 parts: [2] 1) A Signal Peptide that is cleaved in order the rest of the protein to be transported to the outer membrane. 2) The AIDAc is the autotransporter with the transmembrane part. 3) The passeger of AIDA which is the part that is on the exterior side.

We placed as passenger the Tyr1 gene coding to Rhizobium etli tyrosinase Because is the smallest of all tyrosinases (34kDa) and lack cysteines[3] compared to the melA tyrosinase BBa_K193600 used in iGEM before.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1439
  Illegal EcoRI site found at 2135
  Illegal XbaI site found at 1043
  Illegal PstI site found at 1487
  Illegal PstI site found at 2073
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1439
  Illegal EcoRI site found at 2135
  Illegal PstI site found at 1487
  Illegal PstI site found at 2073
  Illegal NotI site found at 1211
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1439
  Illegal EcoRI site found at 2135
  Illegal BglII site found at 471
  Illegal BamHI site found at 454
  Illegal BamHI site found at 2337
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1439
  Illegal EcoRI site found at 2135
  Illegal XbaI site found at 1043
  Illegal PstI site found at 1487
  Illegal PstI site found at 2073
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1439
  Illegal EcoRI site found at 2135
  Illegal XbaI site found at 1043
  Illegal PstI site found at 1487
  Illegal PstI site found at 2073
  Illegal AgeI site found at 533
  
• 1000
  COMPATIBLE WITH RFC[1000]