Coding

Part:BBa_K3570013

Designed by: Anton Mykhailiuk   Group: iGEM20_Toulouse_INSA-UPS   (2020-10-01)
Revision as of 07:29, 13 October 2020 by Antonmykhailiuk (Talk | contribs)


URA3 selection marker


Biology

The URA3 gene, found in the Saccharomyces cerevisiae yeast, encodes a protein called Orotidine-5'-phosphate (OMP) decarboxylase which catalyzes the sixth step in the de novo biosynthesis of pyrimidines, converting OMP into uridine monophosphate (UMP).

URA3 gene serves as a commonly used yeast selectable marker. When inserted into an integrative or replicative plasmid, URA3 allows to counter-select the cells that acquired prototroph character for uracil. Those cells should not have the functional HIS3 gene in its genome[1].

Experiments

Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.

References:

  • [1]- Old, R. W., & Primrose, S. B. (1981). Principles of gene manipulation: an introduction to genetic engineering (Vol. 2). Univ of California Press.
  • [2]- FL38 plasmid
  • [3]- SGD:S000000747

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 278
    Illegal BglII site found at 1374
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None