Part:BBa_K3570008

HIS3 selection marker

Usage

HIS3 gene, found in the Saccharomyces cerevisiae yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyzes the sixth step in histidine biosynthesis(1). It is analogous to hisB in Escherichia coli. HIS3 gene serves as a commonly used yeast selectable marker. When HIS3 gene is inserted into an integrative or replicative plasmid, it selects and maintains a yeast vector in yeast host cells. Those cells should not have the functional HIS3 gene in its genome[1].

The sequence contains HIS3 specific promoter, HIS3 coding sequence, and HIS3 terminator.

Source

• [1]Old, R. W., & Primrose, S. B. (1981). Principles of gene manipulation: an introduction to genetic engineering (Vol. 2). Univ of California Press.
• [2]GenBank: U03439.1
• [3]RS313 plasmid https://www.addgene.org/vector-database/3972/
• [4]https://web.archive.org/web/20060505213339/http://db.yeastgenome.org/cgi-bin/locus.pl?locus=his3

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1005
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 896
  Illegal BglII site found at 956
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]