DNA

Part:BBa_K3570006

Designed by: Anton Mykhailiuk   Group: iGEM20_Toulouse_INSA-UPS   (2020-09-30)
Revision as of 18:12, 12 October 2020 by Antonmykhailiuk (Talk | contribs)


DPP1 upstream homologous sequence

Usage

DPP1 upstream homologous arm part shall be used together with DPP1 downstream part (BBa_K3570007) to form a functional yeast integration locus. When DPP1 up put to 5' of the biobrick together with DPP1 downstream to the 3', the biobrick can be integrated into the S. cerevisiae's genome. It will do homologous recombination within the Diacylglycerol pyrophosphate phosphatase 1 (DPP1) gene.

Caracterisation

Source

S. cerevisiae genome, chromosome IV, DPP1 gene. GenBank: CP046084.1

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 50
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 50
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 50
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 50
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None