Coding

Part:BBa_K3629021

Designed by: Arshia Mostoufi   Group: iGEM20_Calgary   (2020-10-12)
Revision as of 08:10, 12 October 2020 by ArshiaMostoufi (Talk | contribs) (→‎Usage and Biology)


mCherry codon optimized for Yarrowia lipolytica

mCherry coding sequence codon-optimized for Yarrowia lipolytica.

Usage and Biology

mCherry is a monomeric red fluorescent protein that is derived from DsRed of Discosoma. mCherry's optimal excitation wavelength is 586nm and its optimal emission wavelength is 610nm. mCherry could be used to tag components and proteins within the cell which can then be visualized using fluorescence spectroscopy and microscopy.

Design

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[edit]
Categories
//awards/part_collection
//cds/reporter/rfp
//chassis/eukaryote/yeast
//classic/reporter
Parameters
None