Coding

Part:BBa_K3629006:Design

Designed by: Sravya Kakumanu   Group: iGEM20_Calgary   (2020-10-11)
Revision as of 05:21, 12 October 2020 by Sravyakakumanu (Talk | contribs)


Neurospora crassa CBHI with Myc Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 585
    Illegal BglII site found at 945
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1453
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for expression and function in Yarrowia lipolytica.

Source

From the genomic sequence of N. crassa, but taken from the paper by Guo et al (2017).

References