Coding

Part:BBa_K3629007:Design

Designed by: Sravya Kakumanu   Group: iGEM20_Calgary   (2020-10-12)
Revision as of 05:20, 12 October 2020 by Sravyakakumanu (Talk | contribs)


Trichoderma reesei CBHII with FLAG tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 341
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 601
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Optimized for expression and function in Yarrowia lipolytica.


Source

From genomic sequence of T. reesei.

References