Coding

Part:BBa_K3629005:Design

Designed by: Sravya Kakumanu   Group: iGEM20_Calgary   (2020-10-11)
Revision as of 05:09, 12 October 2020 by Sravyakakumanu (Talk | contribs)


Modified Penicillium funiculosum CBHI with 6X His tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 564
    Illegal BamHI site found at 1048
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 46
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Optimized for expression and function in Yarrowia lipolytica.


Source

Modified genomic sequence of cellobiohydrolase I from Penicillium funiculosum.

References